منابع مشابه
Moniliform deformation of retinal ganglion cells by formaldehyde-based fixatives.
Protocols for characterizing cellular phenotypes commonly use chemical fixatives to preserve anatomical features, mechanically stabilize tissue, and stop physiological responses. Formaldehyde, diluted in either phosphate-buffered saline or phosphate buffer, has been widely used in studies of neurons, especially in conjunction with dyes and antibodies. However, previous studies have found that t...
متن کاملLiquefied Wood as a Partial Substitute of Melamine-urea- Formaldehyde and Urea-formaldehyde Resins
Maritime pine (Pinus pinaster) sawdust was used to produce liquefied wood by the polyhydric method with acid catalysis. The process was optimized to produce the highest amount of liquefied wood. Wood liquefied at 160oC for 90 min was used in the adhesion tests. The bond strength of veneer glued with ureaformaldehyde and melamine-urea-formaldehyde resins and several mixtures of liquefied wood wi...
متن کاملAssessment of morphology, antigenicity, and nucleic acid integrity for diagnostic thyroid pathology using formalin substitute fixatives.
BACKGROUND With the advent of the formaldehyde standard law in France, and because of the impact of new methods for diagnosis and prognosis in pathology, formalin replacement in surgical pathology laboratories is currently being discussed in France. However, a set of criteria must be assessed before introducing a formalin substitute fixative. The objective of this study was to compare formalin ...
متن کاملEvaluation of two commercial and three home-made fixatives for the substitution of formalin: a formaldehyde–free laboratory is possible
BACKGROUND Formaldehyde (HCHO) is a gas (available as a 37% concentrated solution, stabilized with methanol). The 10% dilution (approximately 4% formaldehyde) has been used as a fixative since the end of the 19th century. Alternative fixatives are also commercially available or may be prepared in-house in laboratories. Statements by the IARC, along with other USA agencies (CalEPA, RoC/NTP) on t...
متن کاملEffects of Different Fixatives on -Galactosidase Activity
Galactosidase ( -Gal) staining is widely used to demonstrate specific gene expression during evaluation of gene targets in vivo. This technique is extremely sensitive to fixation. Optimal fixation conditions are necessary to obtain the maximal -Gal activity. In this experiment, Carnoy’s and three different aldehyde fixatives were used at different temperatures and over different time points. Ki...
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ژورنال
عنوان ژورنال: American Journal of Clinical Pathology
سال: 2011
ISSN: 1943-7722,0002-9173
DOI: 10.1309/ajcphh1b0cocbgom